The U.S. Embassy in Moscow says it has raised human rights concerns with the Foreign Ministry's ombudsman.
The embassy, in a tweet, said it had expressed its concern over Russia's handling of the violence in Bolotnaya Square in May 2012, its law on foreign funding of NGOs, the rights of sexual minorities in the country, and foreign adoptions. No more details were available.
Twelve protesters in an antigovernment rally on Bolotnaya Square were put on trial in June for taking part in mass unrest.
Activists said ensuing clashes were planned provocations by the authorities, while a law passed in 2012 requires NGOs receiving outside funding to register as "foreign agents."
In addition, a 2013 Russian law forbids dissemination of "homosexual propaganda" to minors.
Russia has also prohibited U.S. couples from adopting Russian orphans.
With reporting by Interfax