Dhafir al-Ani, a spokesman for the Iraqi Accordance Front, said the bloc has informed Iraq's main Shi'ite alliance of its decision to reject al-Ja'fari.
The Kurdish alliance has also made a definitive decision to reject al-Ja'fari.
The Shi'ite United Iraqi Alliance (UIA) is due to hold internal talks on April 10 and to consult with Kurdish and Sunni parties on the al-Ja'fari issue, which has deadlocked the formation of a government of national unity. It is now four months since parliamentary elections.
A three-member Shi'ite committee has been formed to discuss the impasse.
Sunnis and Kurds blame al-Ja'fari for doing little to end sectarian violence between Sunnis and Shi'a.
(compiled from agency reports)