Patrushev said those responsible for organizing the 2004 Orange Revolution in Ukraine are now preparing a similar revolt in Belarus.
He named the Peace Corps -- which pulled out of Russia in 2003 amid FSB spying allegations -- as well as the British medical charity Merlin, the Saudi Red Crescent and a Kuwaiti group he called the Society of Social Reforms.
In Washington, Peace Corps spokeswoman Barbara Daly dismissed Patrushev's charges as "completely baseless, without merit and not true." Merlin also denied the allegations.
Patrushev also accused the International Republican Institute, a U.S. pro-democracy group, of earmarking $5 million to finance the Belarusian opposition this year.