The comments by government spokesman Gholam-Hussein Elham are the latest condemnation of the film in Tehran, where officials have called it insulting to Iranians.
A group of members of parliament have urged Foreign Minister Manuchehr Mottaki and Culture and Islamic Guidance Minister Mohammad Hussein Saffar-Harandi to ask other Muslim countries not to show the "anti-Iranian" movie.
The film tells the story of the battle of Thermopylae, in which 300 Spartan warriors led by King Leonidas heroically fought off an attack by a massive Persian army.
The film depicts the Persian army as ruthless but repeatedly outsmarted by the Greeks, who are only defeated in the end by treachery.
(IRNA, Mehr, Reuters)